Streptozotocininduced diabetes in the rat is associated with. Improving the reliability and utility of streptozotocininduced rat. Stz induced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. The most usual substances to induce diabetes in the rat are alloxan and streptozotocin. Role of nicotinamide in streptozotocin induced diabetes in animal models. The longterm effects of streptozotocin and possible recovery from streptozotocin induced diabetes are explored much less. The polyherbal extract contains the whole plant extract of adiantium capillus, seeds of astercantha longifolia, fruits of callicarpa macrophylla, bark of ficus benghalensis, aerial parts of melia azedarach. Clinicopathological effect of extract on streptozotocin. Induction of diabetes by streptozotocin in rats springerlink. Therefore, the aims of the present study were to assess 1 the effects of glucose and insulin on vsmc proliferation and migration in vitro, 2 response to balloon injury in rats with streptozotocin stz induced diabetes with or without adjunct insulin therapy, and 3 neointimal hyperplasia after vascular injury in nondiabetic rats with.
Streptozotocininduced diabetic ratsmice stz model advantages. Sonnenblick, and james scheuer summary in order to determine whether diabetic cardiomyopathy in rats. Oxidative stress parameters, namely, serum superoxide dismutase, catalase, malondialdehyde and fructosamine in diabetic rats were reverted to respective normal values after glycyrrhizin administration. Characterisation of streptozotocin induced diabetes mellitus. Diabetes was associated with significantly increased activities of catalase cat, glutathione reductase gssgrd, and cuznsuperoxide dismutase sod in the pancreas and of cat and gssgrd in the heart. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. The mechanism of alloxan and streptozotocin action in b. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. To determine whether myocyte death and angiotensin ii at ii formation are implicated in the development of diabetic cardiomyopathy, rats were injected with streptozotocin, and apoptosis and. In control and streptozotocin treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Antihyperglycemic effect of ginkgo biloba extract in.
One hourthirty minutes after injection of stz, all animals received an intraperitoneal injection of glucose 33% to overcome hypoglycemia fatal in rats induced by stz after the pancreatic. Murugesan2 1department of biochemistry, kanchi shri krishna college of arts and science, kilambi. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. This suggested the drugs use as an animal model of diabetes, and as. Evaluation of antidiabetic activity of ginkgo biloba in. Dioscorea esculenta induced increase in muscle sex steroid hormones is associated with enhanced insulin sensitivity in a type 2 diabetes rat model koji sato,,1 satoshi fujita, and motoyuki iemitsu faculty of sport and health science, ritsumeikan university, kusatsu, japan. Thirty five rats were used in the study, five for each group. Streptozotocin diabetesinduced changes in aorta, peripheral. The aim of this study was to evaluate the antihyperglycemic effects of gbe on streptozotocin stz induced diabetes in rats. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Altered insulin signaling and thermal nociception depend on the duration of streptozotocin induced diabetes in rats defective insulin signaling in defective insulin signaling in peripheral nerve may contribute to diabetic neuropathy. Myocyte death in streptozotocininduced diabetes in rats. Effects of aqueous extract of fadogia agrestis stem in alloxaninduced diabetic rats.
However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. The present study designed to investigate the antioxidant activity of ethanolic extract of a. However, treatment of stz induced diabetes in rats with either water or ethanolic extract of a. We investigated the possible involvement of reactive oxygen radicalrelated processes in chronic 12wk diabetes induced in rats by streptozocin stz. Galangin, a natural flavonoid reduces mitochondrial oxidative damage in streptozotocin induced diabetic rats. In rats with diabetes induced by streptozotocin stz, we. The present study was designed to investigate the characteristics of diabetes mellitus induced by.
Pdf induction of diabetes by streptozotocin in rats. Detailed in this unit are protocols for producing stz. Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. Streptozotocininduced diabetes in laboratory animals presents an experimental model whose value is in the elucidation of causal relationships related to human diabetes mellitus. Effects of balloon injury on neointimal hyperplasia in. Pdf grape seed proanthocyanidin gsp bears a very powerful antioxidant effects. Dec 18, 2007 alloxan has two distinct pathological effects.
The mechanisms of alloxan and streptozotocininduced diabetes. Streptozotocininduced type 1 diabetes in rodents as a. Abnormal cardiac function in the streptozotocininduced, non. Altered insulin signaling and thermal nociception depend on. Stz diabetic rats were treated with oral doses of mebs 200 and 400 mgkg for 8 weeks.
Experimental type 2 diabetes mellitus was induced by neonatal injection. The aim of this study was to determine how the pharmacological reactivity of intrapulmonary arteries is altered in a rat model of diabetes. For this purpose, diabetes was induced by administration of streptozotocin 40 mgkg body weight, intraperitoneally. The present study investigated the possible therapeutic effects of aqueous extract and oil of moringa oleifera seed on certain biochemical markers in streptozotocin induced diabetes mellitus in albino rats. Methods a total of 30 female sd rats were caged with male rats for pregnancy, 27 pregnant rats were obtained and weighed. The full text of this article is available as a pdf 180k.
Body weights and blood glucose levels were monitored weekly. Pdf protective role of grape seed proanthocyanidin antioxidant. May 30, 2006 diabetes is associated with declining sexual function in women. Shaban and muslim ali department of biological sciences, faculty of science, kuwait university, kuwait. The animal experiments were performed according to the guidelines for laboratory animals established by the wuhan university center for animal center experimenta3lab, and all study protocols were subjected to approval by the institutional center of wuhan university wuhan, china. Citrullus colocynthis and bryonia dioica springerlink. The neurological consequences of diabetes mellitus in the brain have gained attention most recently. In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to 70 mgkg rakieten et al. Objective the present study was designed to evaluate the diabetes induced neuronal damage in the hippocampus of the rat brain.
Antidiabetic and hypolipidaemic properties of garlic. T1 effect of streptozotocin induced diabetes on rat hippocampus. Diabetes is associated with declining sexual function in women. At cbi, this model is induced in male bn rats by stz administration. Streptozotocin induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. Alloxaninduced diabetes delays repair in a rat model of. Comparison between the therapeutic effects of differentiated and undifferentiated whartons jelly mesenchymal stem cells in rats with streptozotocin induced diabetes. Anti diabetic property of aqueous extract of stevia. Short and longterm glycemic control of streptozotocininduced. Ameliorative effects of glycyrrhizin on streptozotocin. Rats, weighing 150200 g, fasted overnight were used for induction of diabetes. Background diabetes results in cardiac dysfunction.
Modeling type 2 diabetes in rats using high fat diet and. Streptozotocininduced diabetes lowers retinolbinding. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Effect of bauhinia holophylla treatment in streptozotocininduced. In these models, male wistar rats at 8 weeks of age 200300 g are selected from animal supply facility of sgpgims lucknow, and house in a 12h light, 12h dark cycle environment, temperature 25c, with standard rat diet and water in metabolic cages for one week prior to. Role of nicotinamide in streptozotocin induced diabetes in. The streptozotocin induced stz model of diabetes induced by stz administration in rats serves as a validated and reproducible model of retinal hypervascularity in brown norway rats bn. Skeletal muscle proteolysis was studied in rats 1 day after induction of diabetes with 65 mgkg streptozocin. The data show that diabetic stzrats respond to exogenous insulin with regard to glucose levels as well as kidney parameters and a suitable.
Full text streptozotocininduced type 1 diabetes in. Streptozotocininduced diabetes disrupts the body temperature. Streptozotocininduced diabetic models in mice and rats. Streptozotocin stz induced diabetic stzd rats exhibit variable alterations in thermal nociceptive responses. Blood glucose level and lipid profile of streptozotocin. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis. The diabetic rats gained 83% less weight despite consuming 45% more feed than the nondiabetic controls. Research article in streptozotocin induced diabetic rats. Streptozotocin use in mice and rats version 1 date of publication. Characterisation of streptozotocin induced diabetes mellitus in swiss albino mice 11sachin arora, shreesh kumar ojha and divya vohora2 2department of pharmacology, faculty of pharmacy, jamia hamdard, new delhi, india abstract. The objective of this study was to evaluate the potential effect of sodium alginate from sargassum crassifolium on glucose level and lipid profile in streptozotocininduced diabetes rats. Animalsmale, portonwistar rats were obtained from sac1 ltd.
Effects of streptozotocininduced type i diabetes mellitus on. Antidiabetic activity of two aqueous extracts of two cucurbitaceae. From the above results it is shown that the treatment. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. The present study was designed to evaluate the diabetes induced neuronal damage in the hippocampus of the rat brain. Pathophysiological changes of the lower urinary tract. Streptozotocin stz is a widely used chemical for the induction of experimental diabetes in rodents. Type 2 diabetes was induced by feeding rats with hf diet 55% fat for 5 weeks and a single dose of stz 35 mgkg i.
Streptozotocininduced diabetes in the rat is associated. To induce type ii diabetes mellitus, rats were injected with stz intraperitoneally at a dose of 45 mg kg. Streptozotocin diabetesinduced changes in aorta, peripheral nerves and stomach of wistar rats r. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Neonatal streptozotocininduced rat model of type 2 diabetes. Diabetes was induced in group 4, group 5 and group 6 rats by subjecting them to 10% fructose water over a period of 2 weeks, followed by administration of a single intraperitoneal injection of 40 mgkg b. Changes in oxidative stress and antioxidant enzyme. Methods diabetes was induced in 8 week old male wistar rats n96 using a single rapid injection.
Pdf effect of streptozotocin induced diabetes on rat. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. Biomimetic carbon monoxide nanogenerator ameliorates. Streptozotocininduced experimental diabetes in male wistar rats. Induction of diabetes by streptozotocin in rats ncbi nih.
After treatment, in the pancreas no insulitis was observed. Effects of glutamine supplementation on oxidative stressrelated gene expression and antioxidant properties in rats with streptozotocin induced type 2 diabetes volume 107 issue 8 peihsuan tsai, junjen liu, chuili yeh, wanchun chiu, sungling yeh. Effects of streptozotocin induced type i diabetes mellitus on the sinoatrialnode of the rat heart yu zhang1, yanwen wang1, joseph yanni1, mohammed anwar qureshi2, xue cai1, natalie gardiner1, frank christopher howarth2, halina dobrzynski1 1institute of cardiovascular sciences, university of manchester. Streptozotocin is a glucosaminenitrosourea compound.
Pdf in the present study crude powder and various extracts of teucrium. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. In our experiments, we followed the rats with streptozotocin induced diabetes for one year and a recovery was observed in a significant portion of animals. At 99g body weight, diabetes was induced in some of the rats by intravenous administration of streptozotocin a gift of upjohn ltd. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Retinolbinding protein rbp and transthyretin ttr in the plasma, liver and kidney, retinol in plasma, and total vitamin a in the liver were measured in rats 6 weeks after diabetes mellitus had been induced by streptozotocin stz. Three days after the stz injection, the blood was withdrawn from the tail vein, and the glucose level was determined. We evaluated pathophysiological characteristics of the lower urinary tract dysfunction in a streptozotocin stz induced diabetic rat model. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells.
Characterisation of pain responses in the high fat diet streptozotocin model of diabetes and the analgesic effects of antidiabetic treatments. Dioscorea esculenta induced increase in muscle sex steroid. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. Pdf comparative hypoglycemic activity of different extracts of. Progression of early phase diabetic nephropathy in. Effect of streptozotocin diabetes and insulin treatment on. In mammals, the temperature rhythm is regulated by the circadian pacemaker located in the suprachiasmatic nuclei, and is considered amarker rhythm. Antidiabetic and antihyperlipidemic effect of balanites aegyptiaca.
Results of this preliminary study might suggest a beneficial effect of using hadsc for the treatment of stz induced diabetes in adult nude rats. Stz is well known to cause pancreatic bcell damage, whereas na is administered to rats to partially protect insulinsecreting cells against stz. Antidiabetic effects of hesperidin and naringin in type 2 diabetic rats osama m. For induction of experimental diabetes in male adult rats weighted 250300 grams 7590 days. Role of insulin, oxidative stress and nitric oxide nagy m a1, bastawy m a1, 2abdelhamid n m, 1chemistry department, faculty of science, benisuef university, egypt 2biochemistry department, faculty of pharmacy, miniauniversity, egypt. The activities of ast and alt and the level of bilirubin were increased in diabetic rats. Combined treatment with naringin and vitamin c ameliorates. In the present study, we report the effects of induced diabetes and its treatment with or without oral hypoglycaemic drugs on the morphology and. Gajdosikova institute of experimental pharmacology, slovak academy of sciences, bratislava, slovakia abstract. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. The present study was an attempt to investigate the effect of polyherbal extract on streptozotocin induced diabetes in wistar rats. Streptozotocininduced diabetes disrupts the body temperature daily rhythm in rats angela m ramoslobo, daniella c buonfiglio and jose cipollaneto abstract background. Antidiabetic effect of betulinic acid on streptozotocin.
Diabetes was induced in male wistar rats by the administration of stz 60 mgkg b. Rats treated with stz and na manifest symptoms of type 2 diabetes, while animals with stzinduced type. An evisceration procedure, including functional hepatectomynephrectomy, was performed, and the rate of proteolysis in the remaining tissues, primarily skeletal muscles, was evaluated over 2 h. To explore the effect of okra extract on gestational diabetes mellitus gdm rats and its probable molecular mechanism. Hypoglycemic and hypolipidemic effect of seed hydromethanolic extract of tamarindus indica l. Background diabetes mellitus is an endocrine metabolic disorder of impaired carbohydrate, fat and protein metabolisms characterized by chronic hyperglycemia. Diabetes mellitus presents a major challenge to healthcare systems around the world. Stz induced diabetes are commonly performed in male wistar kyoto rats. Progression of early phase diabetic nephropathy in streptozotocininduced diabetic rats. The mechanism of alloxan and streptozotocin action in b cells. The current research was carried out to explore the antidiabetic effect of aqueous extract of stevia rebaudiana leaves in albino rats.
Possible therapeutic effects of vindoline on testicular. Abd elbaky department of clinical pathology, faculty of veterinary medicine, cairo university, giza, egypt abstract. Streptozotocininduced diabetic neuropathy in mice and rats. Administration of both streptozotocin stz and nicotinamide na has been proposed to induce experimental diabetes in the rat. Diabetes was induced by stz injection into male, 6 to 23 weeks old, sprague dawley rats. Clinicopathological effect of extract on streptozotocin induced diabetes in rats abeer a. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water.
Effects of glutamine supplementation on oxidative stress. Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. A comparison with the chronic changes in human diabetes will indicate whether the stz diabetic rat is an adequate model of the human disease. Alloxan streptozotocin pancreatic b cells mechanism of action diabetes the induction of experimental diabetes in the rat using chemicals which selectively destroy pancreatic b cells is very convenient and simple to use. In our present study, metabolic changes and insulin secretion were investigated in rats with diabetes induced by administration of 60 mg of stz and 90 mg of na per kg body weight. The ginkgo biloba extract gbe has been reported to have a wide range of health benefits in traditional chinese medicine.
Ameliorative potential of morin in streptozotocin induced neuropathic pain in rats. Streptozotocininduced type 1 diabetes in rodents as a model. In this study, diabetes was induced by a single injection of streptozotocin stz, 45 mgkg, i. Full text streptozotocininduced type 1 diabetes in rodents. A comparison with the chronic changes in human diabetes will indicate whether the stzdiabetic rat is an adequate model of the human disease. The effect of streptozotocininduced diabetes in rats on. Streptozotocinnicotinamideinduced diabetes in the rat. Changes in oxidative stress and antioxidant enzyme activities. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Metabolic disturbances and defects in insulin secretion in.
Jun 08, 2009 secondly, i am little confused as to how these sulfonylureas can help in stz induced diabetes in rats as their mechanism of action is based on extra release of insulin, and stz kills the pancreatic cells. Stz 60 mgkg was injected intraperitoneally into male. Therapeutic effect of okra extract on gestational diabetes. Methods diabetes was induced in 8 week old male wistar rats. Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic.
Because decreased no contributes to abnormal vascular function in diabetics, we hypothe. In the mid1960s, streptozotocin was found to be selectively toxic to the beta cells of the pancreatic islets, the cells that normally regulate blood glucose levels by producing the hormone insulin. Scribd is the worlds largest social reading and publishing site. The effect of streptozotocin induced diabetes in rats on cardiac contractile proteins ashwani malhotra, somsong penpargkul, frederick s.